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81.
82.
Jelena Rajkov Athimed El Taher Astrid Bhne Walter Salzburger Bernd Egger 《Molecular ecology》2021,30(1):274-296
Variation in gene expression contributes to ecological speciation by facilitating population persistence in novel environments. Likewise, immune responses can be of relevance in speciation driven by adaptation to different environments. Previous studies examining gene expression differences between recently diverged ecotypes have often relied on only one pair of populations, targeted the expression of only a subset of genes or used wild‐caught individuals. Here, we investigated the contribution of habitat‐specific parasites and symbionts and the underlying immunological abilities of ecotype hosts to adaptive divergence in lake–river population pairs of the cichlid fish Astatotilapia burtoni. To shed light on the role of phenotypic plasticity in adaptive divergence, we compared parasite and microbiota communities, immune response, and gene expression patterns of fish from natural habitats and a lake‐like pond set‐up. In all investigated population pairs, lake fish were more heavily parasitized than river fish, in terms of both parasite taxon composition and infection abundance. The innate immune response in the wild was higher in lake than in river populations and was elevated in a river population exposed to lake parasites in the pond set‐up. Environmental differences between lake and river habitat and their distinct parasite communities have shaped differential gene expression, involving genes functioning in osmoregulation and immune response. Most changes in gene expression between lake and river samples in the wild and in the pond set‐up were based on a plastic response. Finally, gene expression and bacterial communities of wild‐caught individuals and individuals acclimatized to lake‐like pond conditions showed shifts underlying adaptive phenotypic plasticity. 相似文献
83.
Parvana Hajieva Justyna B. Mocko Bernd Moosmann Christian Behl 《Journal of neurochemistry》2009,110(1):118-132
Strong evidence indicates that oxidative stress may be causally involved in the pathogenesis of Parkinson's disease. We have employed human dopaminergic neuroblastoma cells and rat primary mesencephalic neurons to assess the protective potential of three novel bisarylimine antioxidants on dopaminergic cell death induced by complex I inhibition or glutathione depletion. We have found that exceptionally low concentrations (EC50 values ∼20 nM) of these compounds (iminostilbene, phenothiazine, and phenoxazine) exhibited strong protective effects against the toxicities of MPP+ , rotenone, and l -buthionine sulfoximine. Investigating intracellular glutathione levels, it was found that MPP+ , l -buthionine sulfoximine, and rotenone disrupted different aspects of the native glutathione equilibrium, while the aromatic imines did not further influence glutathione levels or redox state on any baseline. However, the imines independently reduced protein oxidation and total oxidant flux, saved the mitochondrial membrane potential, and provided full cytoprotection under conditions of complete glutathione depletion. The unusually potent antioxidant effects of the bisarylimines could be reproduced in isolated mitochondria, which were instantly protected from lipid peroxidation and pathological swelling. Aromatic imines may be interesting lead structures for a potential antioxidant therapy of Parkinson's disease and other disorders accompanied by glutathione dysregulation. 相似文献
84.
Sabine Leonhard-Marek Julia Hempe Bernd Schroeder Gerhard Breves 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2009,179(7):883-896
Hypersecretion of chloride can cause diarrhea, a disease frequently occurring in young pigs, particularly around weaning.
We investigated the contribution of different channels to intestinal Cl− secretion as influenced by age and weaning. Jejunal and colonic epithelia from 4-month-old pigs and 4-week-old piglets were
incubated in Ussing chambers and stimulated by carbachol and forskolin. Changes in short-circuit currents were taken as measure
of electrogenic net Cl− secretion. DIDS or NPPB served to inhibit Ca-activated Cl−-channels and outwardly rectifying Cl−-channels (ORCC) or cystic fibrosis transmembrane regulator (CFTR), respectively. Depolarizing the basolateral membrane allowed
to examine the influence of K+-channels on Cl− secretion. Forskolin-stimulated Cl− secretion was mediated by CFTR. ORCC were not involved. Carbachol-induced Cl− secretion could be ascribed to an enhanced driving force due to the opening of K+-channels, whereas Ca-dependent Cl− channels seemed not to be involved. In jejunum, piglets showed higher Cl− secretion than pigs. Two days after weaning forskolin induced an I
sc overshoot and a faster increase in G
t. In colon, Cl− secretion was neither influenced by age nor by weaning. The data suggest a disposition of porcine jejunum for a higher Cl− secretion in young and freshly weaned piglets, which might be a natural defense mechanism as well as a predisposing factor
for diarrhea. 相似文献
85.
Bernd Heimrich Daniel-Alexander Hesse Yuan-Ju Wu Sonja Schmid Martin Schwemmle 《Cell and tissue research》2009,338(2):179-190
Granule cells are major targets of entorhinal afferents terminating in a laminar fashion in the outer molecular layer of the
dentate gyrus. Since Borna disease virus (BDV) infection of newborn rats causes a progressive loss of granule cells in the
dentate gyrus, entorhinal fibres become disjoined from their main targets. We have investigated the extent to which entorhinal
axons react to this loss of granule cells. Unexpectedly, anterograde DiI tracing has shown a prominent layered termination
of the entorhinal projection, despite an almost complete loss of granule cells at 9 weeks after infection. Combined light-
and electron-microscopic analysis of dendrites at the outer molecular layer of the dentate gyrus at 6 and 9 weeks post-infection
has revealed a transient increase in the synaptic density of calbindin-positive granule cells and parvalbuminergic neurons
after 6 weeks. In contrast, synaptic density reaches values similar to those of uninfected controls 9 weeks post-infection.
These findings indicate that, after BDV infection, synaptic reorganization processes occur at peripheral dendrites of the
remaining granule cells and parvalbuminergic neurons, including the unexpected persistence of entorhinal axons in the absence
of their main targets. 相似文献
86.
87.
88.
Radovan Murín Ghasem Mohammadi Dieter Leibfritz Bernd Hamprecht 《Neurochemical research》2009,34(7):1195-1203
The three essential amino acids, valine, leucine and isoleucine, constitute the group of branched-chain amino acids (BCAAs).
BCAAs are rapidly taken up into the brain parenchyma, where they serve several distinct functions including that as fuel material
in brain energy metabolism. As one function of astrocytes is considered the production of fuel molecules that support the
energy metabolism of adjacent neural cells in brain. Astroglia-rich primary cultures (APC) were shown to rapidly dispose of
the BCAAs, including valine, contained in the culture medium. While the metabolisms of leucine and isoleucine by APC have
already been studied in detail, some aspects of valine metabolism remained to be determined. Therefore, in the present study
an NMR analysis was performed to identify the 13C-labelled metabolites that are generated by APC during catabolism of [U-13C]valine and that are subsequently released into the incubation medium. The results presented show that APC (1) are potently
disposing of the valine contained in the incubation medium; (2) are capable of degrading valine to the tricarboxylic acid
(TCA) cycle member succinyl-CoA; and (3) release into the extracellular milieu valine catabolites and compounds generated
from them such as [U-13C]2-oxoisovalerate, [U-13C]3-hydroxyisobutyrate, [U-13C]2-methylmalonate, [U-13C]isobutyrate, and [U-13C]propionate as well as several TCA cycle-dependent metabolites including lactate.
This article is dedicated to Dr. George DeVries. 相似文献
89.
Jos Luis Riveros Bessie Urquieta Cristian Bonacic Bernd Hoffmann Fernando Bas Gerhard Schuler 《Animal reproduction science》2009,116(3-4):318-325
Plasma concentrations of progesterone (P4), estradiol-17β (E2), estrone (E1) and estrone sulfate (E1S) were measured during gestation in eight guanacos kept in captivity. Gestational length was 346.1 ± 9.8 days. P4 plasma concentrations increased after ovulation and remained elevated until parturition. However, during the last 4 weeks of gestation, a gradual decrease from 4.17 × 1.17±1 nmol/L to 2.02 × 1.95±1 nmol/L on day 5 before parturition was observed, followed by a more abrupt final decline to baseline concentrations which were reached on the day after parturition. Mean E2 plasma concentrations started to increase during the eighth month of gestation, and were significantly elevated up to maximum concentrations of 484.7 × 1.21±1 pmol/L during the last 2 months of pregnancy. Concentrations returned to baseline during the last 2 days of gestation. An increase of E1S concentrations (p < 0.01) was observed in the eleventh month of gestation. Mean E1S concentrations remained rather constant during the last 3 weeks of gestation between 4 to 8 nmol/L until parturition, when a steep precipitous decline was observed. E1 concentrations were slightly elevated during the last 4 weeks of gestation, however, maximum concentrations did not exceed 1.5 nmol/L. The results show distinct species specific features of gestational steroid hormone profiles in the guanaco in comparison to domestic South American camelids, such as a more pronounced gradual prepartal decrease of P4 concentrations prior to the final decline to baseline, and clearly lesser E1S concentrations during the last 4 weeks of gestation, which lack a continuous prepartal increase. 相似文献
90.
Ali Azizi Carola Wagner Bernd Honermeier Wolfgang Friedt 《Plant Systematics and Evolution》2009,281(1-4):151-160
The genus Origanum is often referred to as an under-utilized taxon because of its complex taxonomy. Origanum vulgare L., the most variable species of the genus, is a spice and medicinal herb that is characterized by high morphological diversity (six subspecies). In this study, the relative efficiencies of two PCR-based marker approaches, amplified fragment length polymorphism (AFLP) and selectively amplified microsatellite polymorphic loci (SAMPL), were used for comparable genetic diversity surveys and subspecies discrimination among 42 oregano accessions. Seven assays each of AFLP and SAMPL markers were utilized. Effective multiplex ratio (EMR), average heterozygosity (Hav-p), marker index (MI), and resolving power (RP) of the primer combinations were calculated for the two marker systems. UPGMA and Structure analysis along with PCoA plots derived from the binary data matrices of the two markers depicted the genetic distinction of accessions. Our results indicate that both marker systems are suitable but SAMPL markers are slightly more efficient in differentiating accessions and subspecies than AFLPs. 相似文献